General Cell Based Assays
Sigma Aldrich Fine Chemicals Biosciences Oxalate Assay Kit sufficie
Oxalate or Oxalic Acid is a metabolic breakdown product of the Krebs Cycle in eukaryotes and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator for kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine.
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit, 5 x 96 Assays Bulk Pack, 96-well plate format, colorimetric assay for collagen analysis (conditioned media cells, cell extract, tissue, tissue extract samples)
The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues, in a fast and simple 96-well plate format. This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed. This assay is the only hydroxyproline-based assay that does not need this laborious step, resulting in a fast, sensitive, and easy assay. NOTE: This kit does not contain tubes or a protocol for collagen hydrolysis. If you need these items please order the QuickZyme Total Collagen Assay. Application: conditioned media cells, cell extracts, tissues, tissue extracts. Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many disease related studies.
Molecular Devices cGMP Fluorescent Assay Kit, 96-Well, Bulk, Competitive Immunoassay
CatchPoint cGMP Fluorescent Assay Bulk Kit provides a high-sensitivity, 96-well competitive immunoassay for quantitative measurement of cGMP and guanylate cyclase activity. The bulk format supplies reagents and ten pre-coated plates for high-throughput biochemical and cell-based assays.
- High-affinity reagents optimized for low cGMP levels.
- Ten pre-coated black clear-bottom 96-well plates in bulk format.
- Sufficient reagents for ten 96-well plates (960 reactions at 120 μL per well).
- Competitive immunoassay with a single washing step.
- Flexible read window: 10 minutes to 24 hours after substrate addition.
- Store components at 4°C; unopened kit stable for six months.
Aat Bioquest iFluor™ 750 amine
AAT Bioquest's iFluor™ dyes are developed for labeling proteins, in particular, antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids.
Eurofins Discoverx PathHunter Human P2RY11 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human P2RY11 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure P2RY11 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human NTSR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human NTSR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure NTSR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx InCELL Hunter eXpress MEK1 Kinase Binding Assay, 2pl Kit. (96-0040E15CP0M)
The InCELL Hunter eXpress MEK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of MEK1 via detection of MEK1 levels. This product uses EFC technology. The cell line included overexpresses MEK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology TrKC Rabbit mAb
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family This kinase is a membrane-bound receptor that upon neurotrophin binding phosphorylates itself and members of the MAPK pathway Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position Mutations in this gene have been associated with medulloblastomas secretory breast carcinomas and other cancers Several transcript variants encoding different isoforms have been found for this gene
Eurofins Discoverx PathHunter Human LGR5 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human LGR5 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure LGR5 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.