General Cell Based Assays
Cayman Chemical 20S Proteasome Assay Kit
The 20S proteasome is the proteolytic core of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects, evidence that the proteasome may be an important drug target for the treatment for cancer and inflammatory diseases. Cayman’s 20S Proteasome Assay employs a specific 20S substrate, SUC-LLVY-AMC which, upon cleavage by the active enzyme, generates a highly fluorescent product with an emission wavelength at 480 nm.
Cayman Chemical Cel-Based asy DMF 2.5mL
An acid-sensitive fluorescent probe upon protonation it displays a shift in emission maxima from 410 nm to 510 nm upon excitation at 157 nm has been commonly used in photolithography
Cayman Chemical NonradIoactIve IodID asy K 96
A B2-AR agonist selectively binds to B1- over B2-ARs (Kds 2.9 and 113 nM respectively) as well as B3-adrenergic B2 bradykinin NK1 and NK2 receptors up to 3 M induces cAMP accumulation in cultured human bronchial epithelial cells relaxes guinea pig tracheal strips precontracted with carbamoylcholine ovalbumin or histamine (pD2s 8.4 9.5 and 9.5 respectively) reverses histamine- and ovalbumin-induced bronchoconstriction in guinea pigs (ED50s 1 and 40 nmol/kg respectively)
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-p70 S6K (Thr389) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-p70 S6K (Thr389) Detection Kit, 500 Assay Points
American Research Products Inc Rat regulated on actiVation in
Rat regulated on actiVation in normal T-cell eXpressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit from CUSABIO Detection Range 15 6 pg/mL-1000 pg/mL Sensitivity 7 8 pg/mL Sample serum plasma cell culture supernates tissue homogenates Method Sandwich
Bioassay Systems EnzyChrom™ Aspartate Transaminase Assay Kit. For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.
For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity. Key Features: Sensitive. Linear detection range: 2-100 U/L. Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min. Method: OD340nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 2 U/L.
Eurofins Discoverx PathHunter eXpress CCR5 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0224E2CP0L)
The PathHunter CCR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL3/MIP-1α) based activation of CCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR5 and EA-tagged β-Arrestin-2. Activation of CCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Mitochondrial Membrane Pot
Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores leading to the release of cytochrome c into the cytosol which in turn triggers other downstream events in the apoptotic cascade.
STEMCELL Technologies ImmunoCult™ NK Cell Expansion Coating Material, Size: 1.5 mL
Consistently expand natural killer (NK) cells in serum-free conditions without the use of problematic feeder cells.Use ImmunoCult™ NK Cell Expansion Kit to provide optimized culture conditions for expanding NK cells with high yields. This kit comprises ImmunoCult™ NK Cell Base Medium, ImmunoCult™ NK Cell Expansion Supplement, and ImmunoCult™ NK Cell Expansion Coating Material, providing you with a complete, easy-to-use culture system. After just 14 days in culture, cells can be harvested and used directly in your downstream applications.This kit is compatible with many of our other upstream and downstream products. For example, you can use EasySep™ cell separation kits to isolate NK cells that can then be immediately expanded using ImmunoCult™ NK Cell Expansion Kit.
Cayman Chemical BIlIrubIn asy DMSO 5mL
Bilirubin assay DMSO has been tested and formulated to work exclusively with Cayman’s Total and Direct Bilirubin Colorimetric Assay Kit (Item No. 701720).
Cell Biolabs Inc Total Cholesterol Assay Kit (Fluorometric), 192 assays
Cell Biolabs’ Total Cholesterol Assay Kit is a simple fluorometric assay that measures the amount of total cholesterol present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, cholesterol standards and unknown samples. Sample cholesterol concentrations are determined by comparison with a known cholesterol standard. Cholesteryl esters can be quantified by subtracting the free cholesterol values from the total cholesterol value.
Cell Biolabs Inc Lipid Extraction Kit (Chloroform-Free), 50 preps
Traditional lipid extraction by the Folch method is tedious and uses toxic chloroform as the key solvent. In addition, the extracted lipids end up in the bottom layer which is inconvenient. Cell Biolabs' Lipid Extraction Kit (Chloroform-Free) uses an organic solvent system that results in the organic phase being on the top, making it easier to use while eliminating the need for chloroform. Each kit provides sufficient reagents for 50 preps based on a 100µL sample size.